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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Solos. |
Data corrente: |
06/02/2015 |
Data da última atualização: |
06/02/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. |
Afiliação: |
D. C. A. LEITE, UFRJ; FABIANO DE CARVALHO BALIEIRO, CNPS; C. A. PIRES; BEATA EMOKE MADARI, CNPAF; A. S. ROSADO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; R. S. PEIXOTO, UFRJ. |
Título: |
Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014. |
DOI: |
10.1590/S1517-83822014000100023 |
Idioma: |
Inglês |
Conteúdo: |
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples. MenosMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-... Mostrar Tudo |
Palavras-Chave: |
Comunidades microbianas; Extração de DNA; Índice de pureza do DNA; PCR-DGGE. |
Thesaurus Nal: |
biochar. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117433/1/BJMv45n1a23.pdf
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Marc: |
LEADER 02342naa a2200265 a 4500 001 2007986 005 2015-02-06 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1590/S1517-83822014000100023$2DOI 100 1 $aLEITE, D. C. A. 245 $aComparison of DNA extraction protocols for microbial communities from soil treated with biochar.$h[electronic resource] 260 $c2014 520 $aMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples. 650 $abiochar 653 $aComunidades microbianas 653 $aExtração de DNA 653 $aÍndice de pureza do DNA 653 $aPCR-DGGE 700 1 $aBALIEIRO, F. C. 700 1 $aC. A. PIRES 700 1 $aMADARI, B. E. 700 1 $aROSADO, A. S. 700 1 $aCOUTINHO, H. L. C. 700 1 $aPEIXOTO, R. S. 773 $tBrazilian Journal of Microbiology, São Paulo$gv. 45, n. 1, p. 175-183, 2014.
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Registro original: |
Embrapa Solos (CNPS) |
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Registros recuperados : 26 | |
2. | | PEIXOTO, R. S.; CHAER, G. M.; FRANCO, N.; REIS JUNIOR, F. B. dos; MENDES, I. de C.; ROSADO, A. S. A decade of land use contributes to changes in the chemistry, biochemistry and bacterial community structures of soils in the Cerrado. Antonie van Leeuwenhoek, v. 98, n. 3, p. 403-413, 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agrobiologia; Embrapa Cerrados. |
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4. | | RACHID, C. T. C. de C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; CHAER, G. M.; PICCOLO, M. de C.; ROSADO, A. S. Changes in soil microbial community and nitrogen cycling in mixed plantations of Eucalyptus urograndis and Acacia mangium culminates in soil nitrate increase. INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 14., 2012, Conpenhague, DE. The power of the small: abstracts... Wageningen, NE:The International Society for Microbial Ecology, 2012. ISME14Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agrobiologia. |
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5. | | LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Solos. |
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6. | | PEIXOTO, R. S.; COUTINHO, H. L. C.; MADARI, B.; MACHADO, P. L. O. A.; RUMJANEK, N. G.; VAN ELSAS, J. D.; SELDIN, L.; ROSADO, A. S. Soil aggregation and bacterial community structure as affected by tillage and cover cropping in the Brazilian cerrados. Soil & Tillage Research, Amsterdam, v. 90, n. 1-2, p. 16-28, nov. 2006.Biblioteca(s): Embrapa Agrobiologia. |
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7. | | LEITE, D. C. de A.; BALIEIRO, F. de C.; PIRES, C. de A.; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. da C.; PEIXOTO, R. S. Efeito de diferentes tipos de biomassa carbonizada (biocarvão) nas comunidades de bactérias e fungos associados às rizosferas de plantas de arroz (Oryza sativa) cultivar BRSMG curinga. In: CONGRESSO LATINOAMERICANO DE MICROBIOLOGIA, 21., 2012, Santos. Resumos [...]. São Paulo: Sociedade Brasileira de Microbiologia: Associação Latinoamericana de Microbiologia, 2012.Biblioteca(s): Embrapa Solos. |
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8. | | RACHID, C. T. C. C.; SANTOS, A. L.; PICCOLO, M. C.; BALIEIRO, F. C.; COUTINHO, H. L. C.; PEIXOTO, R. S.; TIEDJE, J. M.; ROSADO, A. S. Effect of sugarcane burning or green harvest methods on the brazilian cerrado soil bacterial community structure. Plos One, v. 8, n. 3, e59342, Mar. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Solos. |
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9. | | FONSECA, E. da S.; PEIXOTO, R. S.; ROSADO, A. S.; BALIEIRO, F. de C.; TIEDJE, J. M.; RACHID, C. T. C. da C. The microbiome of Eucalyptus roots under different management conditions and its potential for biological nitrogen fixation. Microbial Ecology, v. 75, n. 1, p. 183-191, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Solos. |
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10. | | RACHID, C. T. C. C.; BALIEIRO, F. C.; FONSECA, E. S.; PEIXOTO, R. S.; CHAER, G. M.; TIEDJE, J. M.; ROSADO, A. S. Intercropped silviculture systems, a key to achieving soil fungal community management in eucalyptus plantations. Plos One, v. 10, n. 2, Feb. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Solos. |
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12. | | LEITE, D. C. A.; BALIEIRO, F. de C.; LEITE, L. F. C.; MADARI, B. E.; RIBEIRO, A. de F.; PEIXOTO, R. S.; ROSADO, A. S. Integrated crop-livestock-forest system presents a different soil microbial structure in comparison to other land uses. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Meio-Norte. |
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13. | | LEITE, D. C. A.; BALIEIRO, F. de C.; LEITE, L. F. C.; MADARI, B. E.; RIBEIRO, A. de F.; PEIXOTO, R. S.; ROSADO, A. S. Integrated crop-livestock-forest system presents a different soil microbial structure in comparison to other land uses. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Solos. |
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14. | | RACHID, C. T. C. C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; PINHEIRO, Y. A. S.; PICCOLO, M. C.; CHAER, G. M.; ROSADO, A. S. Mixed plantations can promote microbial integration and soil nitrate increases with changes in the N cycling genes. Soil Biology & Biochemistry, v. 66, p. 146-153, Nov. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Solos. |
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15. | | RACHID, C. T. C. C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; PINHEIRO, Y, A. S.; PICOLLO, M. C.; CHAER, G. M. Mixed plantations can promote microbial integration and soil nitrate increases with changes in the N cycling genes. Soil Biology & Biochemistry, v. 66, p. 146-153, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agrobiologia. |
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16. | | TEIXEIRA, L. C. R. S.; YEARGEAU, E.; BALIEIRO, F. de C.; PICCOLO, M. C.; PEIXOTO, R. S.; GREER, C. W.; ROSADO, A. S. Plant and bird presence strongly influences the microbial communities in soils of admiralty bay, maritime antarctica. Plos One, v. 8, n. 6, jun. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Solos. |
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17. | | LEITE, D. C. de A.; BALIEIRO, F. de C.; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. da C.; PEIXOTO, R. S. Quantificação de genes-chave relacionados ao ciclo de nitrogênio microbiano em rizosfera plantas de arroz (Oryza sativa) cultivar BRSMG curinga tratadas com diferentes tipos de biocarvão. In: CONGRESSO LATINOAMERICANO DE MICROBIOLOGIA, 21., 2012, Santos. Resumos [...]. São Paulo: Sociedade Brasileira de Microbiologia: Associação Latinoamericana de Microbiologia, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Solos. |
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18. | | PEIXOTO, R. S.; COUTINHO, H. L. da C.; MADARI, B. E.; MACHADO, P. L. O. de A.; RUMJANEK, N. G.; ELSAS, J. D. van; SELDIN, L.; ROSADO, A. S. Soil aggregation and bacterial community structure as affected by tillage and cover cropping in the Brazilian cerrados. Soil & Tillage Research, Amsterdam, v. 90, n. 1/2, p. 16-28, Nov. 2006.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Agrobiologia; Embrapa Solos. |
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19. | | RACHID, C. T. C. da C.; LEITE, D. C. de A.; CAVALINI, R. M.; PEIXOTO, R. S.; ROSADO, A. S.; BALIEIRO, F. de C.; PICCOLO, M. de C.; COUTINHO, H. L. da C. Estrutura da comunidade bacteriana e agregação do solo sob cerrado e cana-de-açúcar em Goiás. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas, PE. Anais... São Paulo: SBM, 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Solos. |
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20. | | RACHID, C. T. C. da C.; LEITE, D. C. de A.; COUTINHO, H. L. da C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; ROSADO, R. S.; PICCOLO, M. de C. Impacto da cana na comunidade de desnitrificantes, fluxo de N20 e potencial de desnitrificação do solo de cerrado. In: SIMPÓSIO CIENTÍFICO DOS PÓS-GRADUANDOS NO CENA/USP, 2., 2009, Piracicaba, SP. Anais... Piracicaba, SP: USP, 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Solos. |
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Registros recuperados : 26 | |
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